A limit on behavioral plasticity in speech perception.

It is well attested that we perceive speech through the filter of our native language: a classic example is that of Japanese listeners who cannot discriminate between the American /l/ and /r/ and identify both as their own /r/ phoneme (Goto, 1971). Studies in the laboratory have shown, however, that perception of non-native speech sounds can be learned through training (Lively, Pisoni, Yamada, & Tohkura, 1994). This is consistent with neurophysiological evidence showing considerable experience-dependent plasticity in the brain at the first levels of sensory processing (Edeline & Weinberger, 1993; Kraus, et al., 1995; Merzenich & Sameshima, 1993; Weinberger, 1993). Outside of the laboratory, however, the situation seems to differ: we here report a study involving Spanish-Catalan bilingual subjects who have had the best opportunities to learn a new contrast but did not do it. Our study demonstrates a striking lack of behavioral plasticity: early and extensive exposure to a second language is not sufficient to attain the ultimate phonological competence of native speakers

Cloned interstitial stem cells grow as contiguous patches in hydra

The migration of interstitial cells was analyzed during the growth of stem cell clones in vivo. The spatial distribution of cloned cells was analyzed at a time by which extensive migration of interstitial cells could have occurred. All interstitial cell clones were found to form large contiguous patches of cells. The results indicate that there is little migration of large interstitial cells in undisturbed tissue during normal growth. This finding is surprising since numerous grafting experiments have shown extensive migration of these cells. The implications of finding nonrandomly distributed stem cells are discussed

Immunocompetence in Hydra. Epithelial cells recognize self-nonself and react against it

The evolution of effective immunologic defense mechanisms in multicellular organisms involves the ability of host cells to distinguish betweeen self and nonself and to react appropriately to eliminate foreign tissue. By producing interspecies grafts we have obtained evidence that immunorecognition followed by incompatibility reactions occur in Hydra. Our results demonstrate that epithelial cells of Hydra recognize and phagocytose foreign hydra cells, indicating that they are the effector cells in the incompatibility reactions. This observation is consistent with the idea that immunocompetence appeared early in the evolution of multicellular organisms

Male and female stem cells and sex reversal in Hydra polyps

Single interstitial stem cells of male polyps of Hydra magnipapillata give rise to clones that differentiate either male or female gametes. To test the sexual stability of these clones, stem cells were recloned. The results indicate that stem cells from female clones are stable in their sexual differentiation capacity; male stem cells, by comparison, switch sexual phenotype at the rate of 10-2 per cell per generation. As a result, female polyps contain only female stem cells; male polyps contain a mixture of male and female stem cells. A model is presented in which the sexual phenotype of Hydra polyps is controlled by (i) the switching rate of male and female stem cells and (ii) the repression of female differentiation by male stem cells

Growth regulation in Hydra. Relationship between epithelial cell cycle length and growth rate

The relationship between epithelial cell production and growth rate was investigated in Hydra attenuata under different feeding regimes. The increase of epithelial cell number was compared to the duration of the epithelial cell cycle using standard methods of cell cycle analysis. The results indicate that cell cycle changes accompanying changes in feeding regime are not sufficient to explain the altered growth rate. Under heavy feeding regimes, epithelial cell production equals tissue growth rate. At low feeding level or under starvation conditions the epithelial cell cycle lengthens and growth rate of epithelial cell population is slowed. However, the cell cycle changes are insufficient to account for the reduction in tissue growth and thus there is an effective overproduction of epithelial cells amounting to 10% per day. Evidence suggests that these excess cells are phagocytized by neighboring cells in the tissue. Thus phagocytosis is directly or indirectly involved in regulating the growth of hydra tissue

Resolving Architectural Mismatches of COTS Through Architectural Reconciliation

The integration of COTS components into a system under development entails architectural mismatches. These have been tackled, so far, at the component level, through component adaptation techniques, but they also must be tackled at an architectural level of abstraction. In this paper we propose an approach for resolving architectural mismatches, with the aid of architectural reconciliation. The approach consists of designing and subsequently reconciling two architectural models, one that is forward-engineered from the requirements and another that is reverse-engineered from the COTS-based implementation. The final reconciled model is optimally adapted both to the requirements and to the actual COTS-based implementation. The contribution of this paper lies in the application of architectural reconciliation in the context of COTS-based software development. Architectural modeling is based upon the UML 2.0 standard, while the reconciliation is performed by transforming the two models, with the help of architectural design decisions.

Haro15: Is it actually a low metallicity galaxy?

We present a detailed study of the physical properties of the nebular material in multiple knots of the blue compact dwarf galaxy Haro 15. Using long slit and echelle spectroscopy, obtained at Las Campanas Observatory, we study the physical conditions (electron density and temperature), ionic and total chemical abundances of several atoms, reddening and ionization structure. The latter was derived by comparing the oxygen and sulphur ionic ratios to their corresponding observed emission line ratios (the eta and eta' plots) in different regions of the galaxy. Applying direct and empirical methods for abundance determination, we perform a comparative analysis between these regions.Comment: (Poster paper) 2 pages, 2 figure

Role of the cellular environment in interstitial stem cell proliferation in Hydra

The role of the cellular environment on hydra stem cell proliferation and differentiation was investigated by introduction of interstitial cells into host tissue of defined cellular composition. In epithelial tissue lacking all non-epithelial cells the interstitial cell population did not grow but differentiated into nerve cells and nematocytes. In host tissue with progressively increased numbers of nerve cells growth of the interstitial cell population was positively correlated to the nerve cell density. In agreement with previous observations (Bode et al. 1976), growth of the interstitial cell population was also found to be negatively correlated to the level of interstitial cells present. The strong correlation between the growth of the interstitial cell population and the presence of interstitial cells and nerve cells implies that interstitial cell proliferation is controlled by a feedback signal from interstitial cells and their derivatives. Our results suggest that the cellular environment of interstitial cells provides cues which are instrumental in stem cell decision making

Reproducibility of electrical caries measurements: A technical problem?

The currently available instrument for electrical detection of occlusal caries lesions {[}Electronic Caries Monitor (ECM)] uses a site-specific measurement with co-axial air drying. The reproducibility of this method has been reported to be fair to good. It was noticed that the measurement variation of this technique appeared to be non-random. It was the aim of this study to analyse how such a non-random reproducibility pattern arises and whether it could be observed for other operators and ECM models. Analysis of hypothetical measurement pairs showed that the pattern was related to measurements at the high and low end of the measurement range for the instrument. Data sets supplied by other researchers to a varying degree showed signs of a similar non-random pattern. These data sets were acquired at different locations, by different operators and using 3 different ECM models. The frequency distribution of measurements in all cases showed a single or double end-peaked distribution shape. It was concluded that the pattern was a general feature of the measurement method. It was tentatively attributed to several characteristics such as a high value censoring, insufficient probe contact and unpredictable probe contact. A different measurement technique, with an improved probe contact, appears to be advisable. Copyright (C) 2005 S. Karger AG, Basel